Alessandro Florenzi, 25, was stretchered off with five minutes left on the clock in Wednesday’s 3-1 Serie A win over Sassuolo, Francesco Totti taking his place on the pitch.
After undergoing a series of tests, Roma have now announced the midfielder requires surgery.
“Alessandro Florenzi underwent diagnostic tests during the night which revealed he has ruptured the anterior cruciate ligament in his left knee,” a statement on the club’s official website reads.
“Florenzi will undergo surgery on Thursday.”
The Italy international has been a key figure for Roma this campaign, making 13 appearances in all competitions, in which he scored once.