During the closing stages of last weekend’s 4-1 victory over Liverpool, the England international pulled up with a hamstring injury and was immediately substituted by head coach Mauricio Pochettino.
Kane was rested for Wednesday’s EFL Cup fixture with West Ham United, but Pochettino has revealed that he cannot afford to take a risk on the club’s top goalscorer after scans confirmed a minor hamstring strain.
The 45-year-old told reporters: “Not for tomorrow. We cannot take the risk. It’s a minor injury, very small, but we cannot take the risk for tomorrow.
“We will see about Wednesday for Real Madrid but for tomorrow, impossible.”
Kane has scored 17 goals in 16 games for Spurs and England this season.