Netherlands are due to face Belarus and France in the coming days and had hoped to have had Robben involved in some way for the Group A double-header.
The Bayern Munich winger missed a large chunk of last season with adductor problems and has been sidelined in the current campaign with a hamstring strain.
Robben made his first start of the season against Cologne last weekend but it only lasted 45 minutes as he was substituted at half time, with boss Carlo Ancelotti confirming he had suffered a minor rib injury.
“I spoke to Arjen this morning, he’s not coming – he’s not fit and that is unfortunate,” Blind said of the 32-year-old.
“We will have to do without him. That is now clear, and we know where we stand. He does just not feel fit yet. We knew it would be difficult for him.
“We hoped that we could take advantage of that extra quality he brings. Unfortunately that is not the case. We will have to do without him.”
There was better news regarding Wesley Sneijder, though, with the Galatasaray midfielder confident he will be ready despite a thigh issue.
Netherlands opened their qualifying campaign with a 1-1 draw away to Sweden.