Andrew Fifita scored the winning try in Cronulla Sharks’ NRL Grand Final victory over Melbourne Storm on Sunday, but will not travel to England with the Kangaroos.
The 27-year-old prop caused controversy in the 2016 season by wearing an armband during a match displaying a message of support for a friend, convicted one-punch killer Kieran Loveridge.
Parramatta Eels winger Semi Radradra, meanwhile, is facing a court case over domestic violence allegations.
Kangaroos head coach Mal Meninga has selected seven uncapped players, including Sharks Grand Final winners James Maloney and Valentine Holmes.
North Queensland Cowboys centre Justin O’Neill, fullback Matt Moylan of the Penrith Panthers, St George-Illawarra Dragons forward Tyson Frizell, Canberra Raiders prop Shannon Boyd and hooker Jake Friend of Sydney Roosters are the other newcomers.
Raiders pair Joey Leilua and Jarrod Croker were ruled out through injury, while James Tedesco of Wests Tigers narrowly missed out on selection, according to Meninga.
Experienced forwards Corey Parker of Brisbane Broncos and the Sharks’ Paul Gallen were also overlooked.
Storm’s Cameron Smith will captain the team, who face New Zealand in Perth on October 15, before taking on Scotland in Hull on October 28 to start their Four Nations campaign.
Australia squad in full:
Cameron Smith (capt), Darius Boyd, Shannon Boyd, Boyd Cordner, Cooper Cronk, Josh Dugan, Blake Ferguson, Jake Friend, Tyson Frizell, Matt Gillett, Valentine Holmes, Greg Inglis, David Klemmer, James Maloney, Josh Mansour, Trent Merrin, Michael Morgan, Matt Moylan, Justin O’Neill, Josh Papalii, Matt Scott, Sam Thaiday, Johnathan Thurston, Aaron Woods.