The New York Red Bulls edged the Montreal Impact 1-0 to go top of the conference, clinching their place in the post-season.
With that result, Toronto – who were held to a 1-1 draw by the Philadelphia Union – and New York City also secured their spots.
Daniel Royer’s first MLS goal was all Jesse Marsch’s Red Bulls needed.
The Austrian midfielder headed in a Chris Duvall cross on the hour-mark to secure victory for his team.
For Toronto, Justin Morrow scored a second-half equaliser as they earned a point against the Union.
Western Conference leaders Dallas, who have already clinched their spot, drew 0-0 at Real Salt Lake.
Orlando City’s hopes took a hit after a 4-1 loss to DC United, while Mauro Manotas scored a hat-trick as the Houston Dynamo beat the Portland Timbers 3-1.
The Vancouver Whitecaps and Colorado Rapids played out a thrilling 3-3 draw and Sporting Kansas City beat the San Jose Earthquakes 2-1.